Author/Authors :
Safari, Dodi Eijkman Institute for Molecular Biology, Jakarta, Indonesia , Valentiya, Feby Department of Biochemistry - Faculty of Mathematics and Natural Sciences - Bogor Agricultural University, Bogor, Indonesia , Salsabila, Korrie Eijkman Institute for Molecular Biology, Jakarta, Indonesia , Paramaiswari, , Wisiva Tofriska Eijkman Institute for Molecular Biology, Jakarta, Indonesia , Tafroji, Wisnu Eijkman Institute for Molecular Biology, Jakarta, Indonesia , Hammerschmidt, Sven Department of Molecular Genetics and Infection Biology - Interfaculty Institute for Genetics and Functional Genomics, Center for Functional Genomcis of Microbes - Universität Greifswald, Greifswald, Germany , Hadinegoro, Sri Rezeki Department of Child Health - Faculty of Medicine - University of Indonesia/Dr Cipto Mangunkusumo Hospital, Jakarta, Indonesia
Abstract :
Streptococcus pneumoniae produces pili that function as adherence factors to bind to epithelial cells in the human upper respiratory tract. In this study, we investigated the prevalence of pilus islets (PIs) in S. pneumoniae strains carried by healthy children below 5 years of age prior to pneumococcal vaccination in 2012 in Lombok Island, Indonesia. In all, 347 archived S. pneumoniae isolates were screened using polymerase chain reactions for the presence of rrgC and pitB genes representing pilus islet 1 (PI-1) and pilus islet 2 (PI-2), respectively. We found that 40 isolates (11.5 %) contained the PI genes: 5.2% carried both PI-1 and PI-2, and 3.5 and 2.9% carried PI-1 and PI-2, respectively. Furthermore, we found that most of the strains carrying either of the PIs belonged to the vaccine serotypes 19F and 19A and were less susceptible to chloramphenicol and tetracycline.
Keywords :
Streptococcus pneumoniae , pilus islet , Lombok Island , Indonesia
