Author/Authors :
Estevão, Lígia Reis Moura Department of Morphology and Animal Physiology - Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil , Simões, Ricardo Santos Department of Morphology and Genetics - Universidade Federal de São Paulo (UNIFESP), Brazil , Cassini-Vieira, Puebla Department of Morphology and Animal Physiology - Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil , Canesso, Maria Cecilia Campos Department of Morphology and Animal Physiology - Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil , Barcelos, Lucíola da Silva Department of Morphology and Animal Physiology - Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil , Rachid, Milene Alvarenga Department of Morphology and Animal Physiology - Universidade Federal Rural de Pernambuco (UFRPE), Recife-PE, Brazil , Câmara, Cláudio Augusto Gomes da Department of Chemistry - UFRPE, Recife-PE, Brazil , Evêncio-Neto, Joaquim Department of Morphology and Animal Physiology - UFRPE, Recife-PE, Brazil
Abstract :
Purpose:
To investigated the inflammatory, angiogenic and fibrogenic activities of the Schinus terebinthifolius Raddi leaves oil (STRO) on wound healing.
Methods:
The excisional wound healing model was used to evaluate the effects of STRO. The mice were divided into two groups: Control, subjected to vehicle solution (ointment lanolin/vaseline base), or STRO- treated group, administered topically once a day for 3, 7 and 14 days post-excision. We evaluated the macroscopic wound closure rate; the inflammation was evaluated by leukocytes accumulation and cytokine levels in the wounds. The accumulation of neutrophil and macrophages in the wounds were determined by assaying myeloperoxidase and N-acetyl-β-D-glucosaminidase activities. The levels of TNF-α, CXCL-1 and CCL-2 in wound were evaluated by ELISA assay. Angiogenesis and collagen fibers deposition were evaluated histologically.
Results:
We observed that macroscopic wound closure rate was improved in wounds from STRO-group than Control-group. The wounds treated with STRO promoted a reduction in leucocyte accumulation and in pro-inflammatory cytokine. Moreover, STRO treatment increased significantly the number of blood vessels and collagen fibers deposition, as compared to control group.
Conclusion:
Topical application of STRO display anti-inflammatory and angiogenic effects, as well as improvement in collagen replacement, suggesting a putative use of this herb for the development of phytomedicines to treat inflammatory diseases, including wound healing.
Keywords :
Wound Healing , Angiogenesis Modulating Agents , Anti-Inflammatory Agents , Phytotherapy , Skin , Mice
