Author/Authors :
Autry, Adam W Department of Radiology and Biomedical Imaging - University of California - San Francisco, USA , Hashizume, Rintaro Department of Neurological Surgery and Biochemistry and Molecular Genetics - Northwestern University - Chicago, USA , David James, C Department of Neurological Surgery and Biochemistry and Molecular Genetics - Northwestern University - Chicago, USA , Larson, Peder E. Z Department of Radiology and Biomedical Imaging - University of California - San Francisco, USA , Vigneron, Daniel B Department of Radiology and Biomedical Imaging - University of California - San Francisco, USA , Park, Ilwoo Department of Radiology - Chonnam National University Medical School - Gwangju, Republic of Korea
Abstract :
e purpose of this study was to demonstrate the feasibility of using hyperpolarized carbon-13 (13C) metabolic imaging
with [1-13C]-labeled pyruvate for evaluating real-time in vivo metabolism of orthotopic diffuse intrinsic pontine glioma (DIPG)
xenografts. Materials and Methods. 3D 13C magnetic resonance spectroscopic imaging (MRSI) data were acquired on a 3T scanner
from 8 rats that had been implanted with human-derived DIPG cells in the brainstem and 5 healthy controls, following injection of
2.5 mL (100 mM) hyperpolarized [1-13C]-pyruvate. Results. Anatomical images from DIPG-bearing rats characteristically
exhibited T2-hyperintensity throughout the cerebellum and pons that was not accompanied by contrast enhancement. Evaluation
of real-time in vivo 13C spectroscopic data revealed ratios of lactate-to-pyruvate (p < 0.002), lactate-to-total carbon (p < 0.002),
and normalized lactate (p < 0.002) that were signicantly higher in T2 lesions harboring tumor relative to corresponding values of
healthy normal brain. Elevated levels of lactate in lesions demonstrated a distinct metabolic prole that was associated with
inltrative, viable tumor recapitulating the histopathology of pediatric DIPG. Conclusions. Results from this study characterized
pyruvate and lactate metabolism in orthotopic DIPG xenografts and suggest that hyperpolarized 13C MRSI may serve as
a noninvasive imaging technique for in vivo monitoring of biochemical processes in patients with DIPG.
