Author/Authors :
Szigeti, Krisztián Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Hegedus, Nikolett Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Rácz, Kitti Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Horváth, Ildikó Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Veres, Dániel S Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Szöllysi, Dávid Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Futó, Ildikó Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Módos, Károly Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Bozó, Tamás Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Karlinger, Kinga Department of Radiology and Oncotherapy - Semmelweis University - Budapest, Hungary , Kovács, Noémi CROmed Translational Research Centers - Budapest, Hungary , Varga, Zoltán Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary , Babos, Magor Mediso Medical Imaging Systems Ltd - Budapest, Hungary , Budán, Ferenc Department of Public Health Medicine - Medical School - University of Pecs, Hungary , Padmanabhan, Parasuraman Lee Kong Chian School of Medicine - Nanyang Technological University, Singapore , Gulyás, Balázs Lee Kong Chian School of Medicine - Nanyang Technological University, Singapore , Máthé, Domokos Department of Biophysics and Radiation Biology - Semmelweis University - Budapest, Hungary
Abstract :
Te aim of this study was to develop and characterize a nanoparticle-based image-contrast platform which is
biocompatible, chemically stable, and accessible for radiolabeling with 201Tl. We explored whether this nanoparticle enhanced
the T1 signal which might make it an MRI contrast agent as well. Methods. Te physical properties of citrate-coated Prussian
blue nanoparticles (PBNPs) (iron(II);iron(III);octadecacyanide) doped with 201Tl isotope were characterized with atomic force
microscopy, dynamic light scattering, and zeta potential measurement. PBNP biodistribution was determined by using SPECT
and MRI following intravenous administration into C57BL6 mice. Activity concentrations (MBq/cm3
) were calculated from the
SPECT scans for each dedicated volume of interest (VOI) of liver, kidneys, salivary glands, heart, lungs, and brain. Results. PBNP
accumulation peaked at 2 hours afer injection predominantly in the kidneys and the liver followed by a gradual decrease in activity
in later time points. Conclusion. We synthetized, characterized, and radiolabeled a Prussian blue-based nanoparticle platform
for contrast material applications. Its in vivo radiochemical stability and biodistribution open up the way for further diagnostic
applications.
