Title of article :
Detection of Eight Respiratory Bacterial Pathogens Based on Multiplex Real-Time PCR with Fluorescence Melting Curve Analysis
Author/Authors :
Hu, Liuyang Department of Laboratory Medicine - Guangdong Second Provincial General Hospital, China , Han, Bing Department of Laboratory Medicine - Guangdong Second Provincial General Hospital, China , Tong, Qin Shenzhen Longhua District Central Hospital, Shenzhen, China , xiao, Hui Guangzhou Baochuang Biotechnology Co., China , Cao, Donglin Department of Laboratory Medicine - Guangdong Second Provincial General Hospital, China
Abstract :
Background and Objective. Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Streptococcus pneumoniae, Haemophilus influenzae, Staphylococcus aureus, Pseudomonas aeruginosa, and Mycobacterium tuberculosis are primary respiratory bacterial pathogens contributing to morbidity and mortality in developing countries. This study evaluated the diagnostic performance of multiplex real-time PCR with fluorescence melting curve analysis (MCA) assay, which was used to detect eight respiratory bacterial pathogens simultaneously.
Keywords :
Eight Respiratory Bacterial , Pathogens Based , Multiplex Real-Time PCR , Fluorescence Melting
Journal title :
Canadian Journal of Infectious Diseases and Medical Microbiology
