Author/Authors :
Chen, Xu Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Ding, Shuizi Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Lei, Cheng Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Qin, Jieli Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Guo, Ting Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Yang, Danhui Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Yang, Min Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Qing, Jie Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , He, Wenlong Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Song, Min Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Zhang, Yan Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Zeng, Huihui Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Qin, Qingwu Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Yang, Lizhen Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Long, Yingjiao Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Chen, Yan BGI Genomics - BGI-Shenzhen, Shenzhen, China , Ma, Bingyin Tianjin Medical Laboratory - BGI-Tianjin - BGI-Shenzhen, China , Ouyang, Ruoyun Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Chen, Ping Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China , Luo, Hong Department of Pulmonary and Critical Care Medicine - the Second Xiangya Hospital - Central South University, Changsha, China
Abstract :
Background
Metagenomic next-generation sequencing (mNGS) has made a revolution in the mode of pathogen identification. We decided to explore the diagnostic value of blood and bronchoalveolar lavage fluid (BALF) as mNGS samples in pneumonia.
Methods
We retrospectively reviewed 467 mNGS results and assessed the diagnostic performance of paired blood and BALF mNGS in 39 patients with pneumonia.
Results
For bacteria and fungi, 16 patients had culture-confirmed pathogen diagnosis, while 13 patients were culture-negative. BALF mNGS was more sensitive than blood mNGS (81.3% vs. 25.0%, p=0.003), and the specificity in BALF and blood mNGS was not statistically significant different (76.9% vs. 84.6%, p=0.317). For 10 patients without culture test, treatments were changed in 2 patients. For viruses, Epstein-Barr virus was positive in blood mNGS in 9 patients. Human adenovirus was detected in both BALF and blood mNGS in 3 patients.
Conclusion
Our study suggests that BALF mNGS is more sensitive than blood mNGS in detecting bacteria and fungi, but blood also has advantages to identify the pathogens of pneumonia, especially for some viruses.
