Author/Authors :
Song, Ningning Department of Nuclear Medicine - Shanghai General Hospital - Shanghai Jiao Tong University School of Medicine, Shanghai, China , Zhao, Lingzhou Department of Nuclear Medicine - Shanghai General Hospital - Shanghai Jiao Tong University School of Medicine, Shanghai, China , Zhu, Meilin School of Basic Medical Sciences - Ningxia Medical University - Yinchuan - Ningxia, China , Zhao, Jinhua Department of Nuclear Medicine - Shanghai General Hospital - Shanghai Jiao Tong University School of Medicine, Shanghai, China
Abstract :
Triple negative breast cancer (TNBC), the most aggressive breast cancer type, is associated with high mortality and recurrence
rates. An active-targeted strategy based on homing peptides is an eective approach to diagnose and treat cancer as it can deliver
imaging agents or therapeutic drugs into desired tissues and accumulate less into o-target tissues. As a homing peptide, LyP-1 has
shown properties of targeting, internalization, and proapoptosis to TNBC. In the study, we designed a Technetium-99m- (99mTc-)
labeled LyP-1 and investigated its feasibility for targeted single-positron emission computed tomography (SPECT) imaging of
TNBC. The results showed that the LyP-1 peptide had acceptable biocompatibility in the studied concentration range and could
specically bind to TNBC cells in vitro. 99mTc-labeled LyP-1 showed high radiochemical purity and stability and could be used as a
probe for targeted SPECT imaging of TNBC cells in vitro and in a TNBC tumor-bearing mouse model. Our ndings indicate that
this active-targeted strategy has great potential to be developed into a new imaging tool for TNBC diagnosis.
