Author/Authors :
Liu, Chunbao Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Zhou, Jun Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Cheng, Xiaojie Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Xia, Liang Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Zhou, Junfen Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Xu, Shufang Huazhong University of Science and Technology - Wuhan, China , Wang, Yichun Department of Nuclear Medicine - union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Zhang, Yongxue Department of Nuclear Medicine - union Hospital - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China , Lu, Diyu Department of Nuclear Medicine - The Central Hospital of Wuhan - Tongji Medical College - Huazhong University of Science and Technology - Wuhan, China
Abstract :
Vascular cell adhesion molecule-1 (VCAM-1) can be a promising target for colitis study because of its critical role in inflammation
development. Single-chain variable fragment (scFv) antibody presents fast blood clearance when served as an imaging probe. We
applied the probe of 99mTc-scFv-VCAM-1 to colitis rabbit to examine its imaging performance. The colitis model rabbit was
prepared, and a typical inflammatory lesion was confirmed in the colon. The probe of 99mTc-scFv-VCAM-1 was synthesized and
injected into the model animal before imaging examination. Scintigraphy detected colitis lesions in both SPECT planar and
SPECT/CT fused images, with higher target-to-nontarget ratios in the model group (2.71 ± 0.31) than those in the control group
(1.12 ± 0.10). Biodistribution study determined tracer uptake in dierent organs, and autoradiography (ARG) confirmed probe
accumulation in colon lesions. e uptake ratio of the model colon to the control colon was 4.71 ± 0.61 in quantitative analysis of
the ARG regions of interest. Stronger VCAM-1 expression in the model colon than that in the control colon was confirmed by
western blotting and immunohistochemistry. Our imaging study indicates molecular imaging with scFv-VCAM-1 as a promising
way for inflammatory bowel disease diagnosis and evaluation.
