Author/Authors :
Hu, Zhao Department of Cardiology - Kunming Medical University, Kunming, China , Cai, Hong-Yan Department of Cardiology - Kunming Medical University, Kunming, China , Luo, Yun-Yan Department of Cardiology - Kunming Medical University, Kunming, China , Xiao, Jian-Ming Department of Cardiology - Kunming Medical University, Kunming, China , Li, Lin Department of Cardiology - Kunming Medical University, Kunming, China , Guo, Tao Department of Cardiology - Kunming Medical University, Kunming, China
Abstract :
Purpose:
To investigate the impact of different hypoxia reoxygenation (HR) times on autophagy of rat cardiomyocytes (H9C2).
Methods:
Rat cardiomyocytes were randomly divided into normal control group (group A), hypoxia group (group B), 2 h HR group (group C), 12 h HR group (group D), and 24 h HR group (group E). LC3 II/LC3 I was determined via western blotting, and cell viabilities of cardiomyocytes were measured using methyl thiazolyl tetrazolium (MTT) assay.
Results:
Cell viabilities in HR model groups were significantly lower than those of group A (P<0.05). LC3 II/LC3 I levels in groups B to D were significantly higher than those of group A (P<0.05), and group D showed the highest LC3 II/LC3 I levels. Cell viabilities in groups B to D were significantly lower than those of group A (P<0.05), with group D showing the lowest cell viabilities (P<0.05).
Conclusions:
Hypoxia can induce autophagy in rat cardiomyocytes, which can be further activated by reoxygenation; most notable after 12 h. Hypoxia-induced cell injury can be aggravated by reoxygenation. The lowest cell viability was observed at 12 h after reoxygenation; however, cell viability can be recovered after 24 h.
Keywords :
Autophagy , Myocytes , Cardiac , Hypoxia , Rats
