Author/Authors :
Ghazi ، Mona Department of Microbiology - School of Medicine, Infectious Diseases and Tropical Medicine Research Center - Shahid Beheshti University of Medical Sciences , Goudarzi ، Hossein Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences , Goudarzi ، Mehdi Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences , Faghihloo ، Ebrahim Department of Microbiology - School of Medicine - Shahid Beheshti University of Medical Sciences , Zakeri ، Maryam School of Dentistry - Shahid Beheshti University of Medical Sciences , Amini ، Ayda School of Dentistry - Shahid Beheshti University of Medical Sciences , Dayyani ، Farnaz Human Viral Vaccine Department - Razi Vaccine Serum Research Institute - Agricultural Research, Education Extension Organization (AREEO) , Yadegarynia ، Davood Infectious Diseases and Tropical Medicine Research Center - Shahid Beheshti University of Medical Sciences , Dadashi ، Masoud Department of Microbiology - Non-Communicable Diseases Research Center, School of Medicine - Alborz University of Medical Sciences
Abstract :
Background: Multiple risk factors are supposed to progress oral cavity carcinoma and among them, the role of neither bacterial nor viral infections should be underestimated. Despite relentless efforts, the accelerating effects of human papillomavirus (HPV), Epstein–Barr virus (EBV), and Porphyromonas gingivalis (P. gingivalis) on oral cancer has not yet been recognized successfully. Taking advantage of these facts, in this study we evaluated the prevalence of HPV, EBV, and P. gingivalis in oral cavity carcinoma. Materials and Methods: A total of 43 oral cavity cancerous tissues and 29 healthy oral ones were collected from Loghman Hospital, Tehran, Iran, between 2016 and 2018. After DNA extraction, the prevalence of HPV, EBV, and P. gingivalis was evaluated by PCR. Results: There were 53.5 well-differentiated (15 male, 9 female), 41.8% moderate (10 male, 5 female), and 4.7% poor (1 male, 3 female) adenocarcinoma paraffin-embedded tissue samples. PCR analysis has shown that there were 1 HPV (age: 46; moderate adenocarcinoma) and 1 EBV (age: 62; moderate adenocarcinoma) positive in different samples. No P. gingivalis was found and there was not any infected tissue with both EBV and HPV. In 31% of control tissues, blisters were observed and in 51.7% there was no mucus. We did not find any association between age, sex, and HPV, EBV positive samples. Conclusion: As sample size can affect the results of epidemiological and clinical study, and due to the low number of positive samples in this study, we concluded that HPV, EBV, and P. gingivalis may not have a detrimental effect on the progression of oral cancer, but further studies are needed.
Keywords :
EBV , HPV , P. gingivalis , Oral cancer
