Author/Authors :
Costa, Caio César Chaves Faculty of Medicine - UEPA, Belem-PA, Brazil , Pereira, Nathalia Gabay Faculty of Medicine - UEPA, Belem-PA, Brazil , Machado, Anna Luiza Melo Faculty of Medicine - UEPA, Belem-PA, Brazil , Dórea, Mariana Albuquerque Faculty of Medicine - UEPA, Belem-PA, Brazil , Cruz, Rafaella Macêdo Monteiro da Faculty of Medicine - UEPA, Belem-PA, Brazil , Silva, Renata Cunha UEPA, Belem-PA, Brazil , Domingues, Robson José de Souza Department of Morphology and Physiological Sciences - UEPA, Belem-PA, Brazil , Yasojima, Edson Yuzur Universidade do Estado do Pará (UEPA), Belem-PA, Brazil
Abstract :
Purpose:
To evaluate the effects of splenic ischemic preconditioning (sIPC) on oxidative stress induced by hepatic ischemia-reperfusion in rats.
Methods:
Fifteen male Wistar rats were equally divided into 3 groups: SHAM, IRI and sIPC. Animals from IRI group were subjected to 45 minutes of partial liver ischemia (70%). In the sIPC group, splenic artery was clamped in 2 cycles of 5 min of ischemia and 5 min of reperfusion (20 min total) prior to hepatic ischemia. SHAM group underwent the same surgical procedures as in the remaining groups, but no liver ischemia or sIPC were induced. After 1h, hepatic and splenic tissue samples were harvested for TBARS, CAT, GPx and GSH-Rd measurement.
Results:
sIPC treatment significantly decreased both hepatic and splenic levels of TBARS when compared to IRI group (p<0.01). Furthermore, the hepatic and splenic activities of CAT, GPx and GSH- Rd were significantly higher in sIPC group than in IRI group.
Conclusion:
sIPC was able to attenuate hepatic and splenic IRI-induced oxidative stress.
Keywords :
Liver Failure , Reperfusion Injury , Oxidative Stress , Ischemic Preconditioning , Rats
