Author/Authors :
Trevisan, Alexandre Maximiliano Department of Gastroenterology - School of Medicine - Universidade de São Paulo (USP), Brazil , Cogliati, Bruno Department of Pathology - School of Veterinary Medicine and Animal Science - USP, Sao Paulo-SP, Brazil , Homem, Adriana Ribeiro Department of Gastroenterology - School of Medicine - USP, Sao Paulo-SP, Brazil , Aloiav, Thiago Pinheiro Arrais Hospital Albert Einstein, Sao Paulo-SP, Brazil , Aquino, Nelson de School of Medicine - USP, Sao Paulo-SP, Brazil , Moreira, Jairo Marques Hospital Albert Einstein, Sao Paulo-SP, Brazil , Reno, Leonardo da Cruz School of Medicine - USP, Sao Paulo-SP, Brazil , Naumann, Alexandre Moulin School of Medicine - USP, Sao Paulo-SP, Brazil , Galvão, Flavio Henrique Ferreira Department of Gastroenterology - School of Medicine - USP, Sao Paulo-SP, Brazil , Andraus, Wellington Department of Gastroenterology - School of Medicine - USP, Sao Paulo-SP, Brazil , D'Albuquerque, Luiz Augusto Carneiro Department of Gastroenterology - School of Medicine - USP, Sao Paulo-SP, Brazil
Abstract :
Purpose:
To evaluate that Connexin (Cx43) plays a role in lesions after hepatic ischemia/reperfusion (IR) injury.
Methods:
We use Cx43 deficient model (heterozygotes mice) and compared to a wild group. The groups underwent 1 hour ischemia and 24 hours reperfusion. The heterozygote genotype was confirmed by PCR. We analyzed the hepatic enzymes (AST, ALT, GGT) and histology.
Results:
The mice with Cx43 deficiency showed an ALT mean value of 4166 vs. 307 in the control group (p<0.001); AST mean value of 7231 vs. 471 in the control group (p<0.001); GGT mean value of 9.4 vs. 1.7 in the control group (p=0.001); histology showed necrosis and inflammation in the knockout group.
Conclusions:
This research demonstrated that the deficiency of Cx43 worses the prognosis for liver injury. The topic is a promising target for therapeutics advancements in liver diseases and procedures.
Keywords :
GAP junctions , Connexin 43 , Ischemia , Reperfusion , Liver , Cell communication , Mice
