Title of article :
Antiproliferative Properties of Clausine-B against Cancer Cell Lines
Author/Authors :
Zain, Wan Nor I’zzah Wan Mohd Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Nutrition and Dietetics, Malaysia , Rahmat, Asmah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Nutrition and Dietetic Department, Malaysia , Othman, Fauziah Universiti Putra Malaysia - Faculty of Medicine and Health Sciences - Department of Biomedical Science, Malaysia , Yap, Taufiq Yun Hin Universiti Putra Malaysia - Faculty of Science - Department of Chemistry, Malaysia
Abstract :
Background: Clausine B, a carbazole alkaloid isolated from the stem bark of Clausena excavata,was investigated for its antiproliferative activities against five human cancer cell lines: HepG2 (hepaticcancer), MCF-7 (hormone-dependent breast cancer), MDA-MB-231 (non-hormone-dependent breastcancer), HeLa (cervical cancer), and CAOV3 (ovarian cancer).Methods: Chang liver (normal cells) was used as a control. The effect of clausine-B was measuredusing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay.Results: Clausine-B was found to be active (IC50 30 μg/mL) against four of the cancer cell linestested. The IC50 values for these four lines were: 21.50 μg/mL (MDA-MB-231), 22.90 g/ml (HeLa),27.00 μg/mL (CAOV3) and 28.94 μg/mL (HepG2). Clausine-B inhibited the MCF-7 cancer cell line at52.90 μg/mL, and no IC50 value was obtained against Chang liver.Conclusion: It is possible that the phenolic group in clausine-B responsible for the antiproliferativeactivities found in this study
Keywords :
Clausine , Clausena excavata , ethnopharmacology , cell survival , medical sciences
Journal title :
The Malaysian Journal Of Medical Sciences (MJMS)
Journal title :
The Malaysian Journal Of Medical Sciences (MJMS)
