The Study of the Toxic Effect of Acetamiprid on Blood Factors, Protein Total, and Lactobacillus in the Intestine of Cyprinus Carpio

Many pesticides enter aquatic ecosystem after use and act as environmental pollutants. Changes in the biochemical parameters of fish blood serum can be considered as a suitable factor for detecting the effects of toxicity and determining the physiological status of fish exposed to pesticides. Lactic acid bacteria are also the most common types of bacteria present in the digestive tract of fish. These bacteria are effective in treating rotavirus diarrhea, lowering blood sugar, blood pressure and cholesterol, and preventing large and small intestine and liver cancer, inflammatory bowel disease, infections, acute diarrhea, and the growth and proliferation of harmful bacteria. They also strengthen the immune system and help digestion and absorption of minerals and vitamins. In this study, Cyprinus Carpio were exposed to 17, 34, 56, 85 and 113 micrograms per liter of Acetamiprid pesticide for 35 days and its effect on protein, red blood cell, hemoglobin, hematocrit, white blood cell and lymphocyte were studied. The results of biochemical studies revealed that after 35 days that Cyprinus Carpio were exposed to Acetamiprid, the levels of red blood cells, hemoglobin, hematocrit, white blood cells and lymphocytes in fish blood decreased significantly; however, there was a significant increase in total blood protein compared to the control sample. Also, after separating the G + bacilli from the intestine of the fish and examining its anti biogram with different doses of Acetamiprid, it was determined that in all doses we did not see the growth of lactose bacilli. Due to the effect of Acetamiprid on blood biochemical parameters and intestinal bacilli, it seems that the use of this pesticide and its subsequent application to running water can affect the survival of Cyprinus Carpio.