Low-Pressure Radiofrequency Cold Plasma for Disinfection of Gutta-Percha Cones

Different methods have been proposed for rapid disinfection of gutta-percha (GP) cones. This study aimed to assess the efficacy of low-pressure radiofrequency cold plasma (LRFCP) in disinfection of GP cones compared to three chemical disinfectants. Materials and Methods: Seventy GP cones were allocated to seven groups of 10 each. All samples were initially sterilized with ethylene oxide (EO) and subsequently inoculated with Staphylococcus aureus (S. aureus), except for the negative control group (n=10). In the experimental groups (n=50), samples were subjected to oneminute chemical disinfection [5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 10% Deconex® 53 PLUS) or LRFCP (30 seconds or one minute). The effectiveness of disinfection was evaluated by counting the colony-forming units (CFUs). Data were analyzed using Kruskal-Wallis test (P=0.05). Result: All experimental groups effectively eliminated S. aureus. LRFCP and 5.25% NaOCl were the most effective agents in disinfection of GP cones. In addition, 2% CHX was significantly weaker than the other agents (P<0.05). Although Deconex® 53 PLUS was less potent than LRFCP groups and NaOCl (P>0.05), it showed higher antibacterial activity than 2% CHX (P>0.05). Conclusion: LRFCP can be assumed as a noninvasive and efficient method for disinfection of GP cones.