Author/Authors :
Kasraeian, Maryam Department of Obstetrics and Gynecology - Maternal Fetal Medicine Research Center - Perinatology Ward - Shiraz University of Medical Sciences, Shiraz, Iran , Ghasemi, Elahe Department of Obstetrics and Gynecology - Maternal Fetal Medicine Research Center - Perinatology Ward - Shiraz University of Medical Sciences, Shiraz, Iran , Dianatpour, Mehdi Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Tanideh, Nader Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Razeghian Jahromi, Iman Cardiovascular Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Khodabandeh, Zahra Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Dorvash, Mohammad Reza Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Zare, Shahrokh Stem Cells Technology Research Center - Shiraz University of Medical Sciences, Shiraz, Iran , Koohi Hosseinabadi, Omid Central Lab - Shiraz University of Medical Sciences, Shiraz, Iran , Tamadon, Amin The Persian Gulf Marine Biotechnology Research Center - the Persian Gulf Biomedical Sciences Research Institute - Bushehr University of Medical Sciences, Bushehr, Iran
Abstract :
Background: Animals can play an important role in preparing tissues for human through
the development of xenotransplantation protocols. The most common problem with
liver transplantation like any other organ transplantation is organ supply shortage.
Objective: To evaluate the in utero xenotransplantation of mouse bone marrow-derived
stromal/stem cells (BMSCs) to the liver of rat fetus to produce mouse liver tissue.
Materials and Methods: BMSCs were isolated and confirmed from enhanced green
fluorescent protein (eGFP)-genetic labeled mice. Using a microinjection protocol, mice
BMSCs were injected into the liver of rat fetuses in utero on day 14 of pregnancy. After
birth, livers were collected and the presence of mice eGFP-positive cells in rat livers
was evaluated through polymerase chain reaction.
Results: The eGFP mRNA was detected in the liver of injected infant rats. BMSCs of
adult mice were capable to remain functional probably as hepatocyte-like cells in liver
of infant rats after in utero xenotransplantation.
Conclusion: BMSCs have the potential for intrauterine xenotransplantation for the
treatment of liver dysfunction before birth. This method can also be used for
xenoproduction of liver tissue for transplantation.
Keywords :
Xenotransplantation , Liver , Bone marrow , Stromal/stem cell , Murine
