Author/Authors :
Hasani, Sahar Student Research Center - Hamadan University of Medical Sciences, Hamadan, Iran , Ghasemi, Hassan Department of Clinical Biochemistry - Abadan School of Medical Sciences, Abadan, Iran , Ranjbar, Akram Department of Toxicology and Pharmacology - School of Pharmacy - Hamadan University of Medical Sciences, Hamadan, Iran , Ghahremani, Reza Department of Exercise Physiology - Faculty of Sport Sciences - University of Birjand, Birjand, Iran , Heidarianpour, Ali Department of Exercise Physiology - Faculty of Physical Education and Sport Sciences - Bu-Ali Sina University, Hamadan, Iran , Abotalebian, Hadis Research Center for Biochemistry and Nutrition in Metabolic Diseases - Kashan University of Medical Sciences, Kashan, Iran , Gharib, Alireza Neurophysiology Research Center - Hamadan University of Medical Sciences, Hamadan, Iran , Kheiripour, Nejat Research Center for Biochemistry and Nutrition in Metabolic Diseases - Kashan University of Medical Sciences, Kashan, Iran
Abstract :
Introduction: In this study, the effect of zinc sulfate (ZS) supplement and eight-week continuous training (CT) and interval training (IT) on liver oxidative stress of morphine-dependent rats following withdrawal syndrome (WS) were evaluated.
Methods: Seventy Wistar rats were randomly divided into seven groups: control rats, withdrawing rats (WS), withdrawing rats receiving 9mg/kg ZS orally (WS+Z), withdrawing rats under CT (WS+CT), withdrawing rats under IT (WS+IT), withdrawing rats under CT and receiving 9mg/kg zinc sulfate (WS+Z+CT), withdrawing rats under IT and receiving 9mg/kg zinc sulfate (WS+Z+IT). Animals were addicted by 0.4g/l morphine sulfate in 21 days. Animals in the training groups ran on a treadmill and received ZS 5 days/week for 8 weeks. At the end of the study, oxidative stress in liver tissue and liver enzymes were measured by spectrophotometric and ELISA methods.
Results: ZS supplement, CT/IT led to decrease DNA damage and malondialdehyde in comparison with morphine group. Also, ZS, CT and IT significantly elevated levels in superoxide dismutase, catalase activity, total antioxidant capacity and thiol groups in the liver of rats in comparison with morphine group. Additionally, it is observed that ZS and CT/IT made a significant reduction in aspartate aminotransferase levels in comparison to the morphine group.
Conclusion: CT/IT with ZS because of its antioxidant effects has the potential to be used for decreased withdrawal syndrome complications.
Keywords :
Zinc Sulfate , Continuous Training , Interval Training , Morphine , Withdrawal Syndrome , Liver
