Effect of Different High-Fat and Advanced Glycation End-Products Diets in Obesity and Diabetes-Prone C57BL/6 Mice on Sperm Function

Background: We aimed to compare the effects of using highfat (HF) and advanced glycation endproducts (AGEs) containing diets to induce obesity and diabetes on sperm function in mice. Materials and Methods: In this experimental study, twenty-five 4-week old C57BL/6 mice were divided into 5 groups and were fed with control, 45% HF, 60% HF, 45% AGEsHF, or 60% AGEsHF diet. After 28 weeks, fast blood sugar, glucose intolerance, insulin concentration, homeostatic model assessments (HOMA) for insulin resistance (IR) and HOMA for beta cells (HOMA beta) from systematic blood were assessed. In addition, body weight, morphometric characteristics of testes, sperm parameters, DNA damage (AO), protamine deficiency (CMAA3), and sperm membrane (DCFHDA) and intracellular (BODIPY) lipid peroxidation were measured. Results: Body mass and fasting blood sugar increased significantly in all experimental groups compared to the control group. Insulin concentration, glucose intolerance, HOMA IR, and HOMA beta were also increased significantly with higher levels of fat and AGEs in all four diets (P 0.05). The changes in the 60% HFAGEs group, however, were more significant (P 0.001). Morphometric characteristics of the testis, sperm concentration, and sperm morphology in the diet groups did not significantly differ from the control group, while sperm motility and DNA damage in the 45%HF were significantly low. Although for protamine deficiency, both 60% HFAGEs and 45% HF showed a significant increase compared to the control, the mean of sperm lipid in the 45% HF group and intracellular peroxidation in the 60% HF-AGEs group had the highest and the lowest increases, respectively. Conclusion:Our results, interestingly, showed that is the negative effects of a diet containing AGEs on examined parameters are less than those in HF diets. One possible reason is detoxification through the activation of the protective glyoxalase pathway as the result of the chronic AGEs increase in the body.