Title of article :
Functional and Flow Cytometric Analysis of Buffalo Cryopreserved Spermatozoa: Comparison of Different Breeds and Incubation Times
Author/Authors :
Rezaei Topraggaleh ، Tohid Department of Anatomical Sciences - Faculty of Medicine - Urmia University of Medical Sciences , Numan Bucak ، Mustafa Department of Reproduction and Artificial Insemination - Faculty of Veterinary Medicine - Selcuk University , Shahverdi ، Maryam Department of Embryology - Royan Institute for Reproductive Biomedicine, Reproductive Biomedicine Research Center - Academic Center for Education, Culture and Research (ACECR) , Koohestani ، Yegane Department of Anatomical Sciences - Faculty of Medicine - Guilan University of Medical Sciences , Furkan Batur ، Ali Department of Urology - Selcuk University School of Medicine , Rahimizadeh ، Pegah Department of Embryology - Royan Institute for Reproductive Biomedicine, Reproductive Biomedicine Research Center - Academic Center for Education, Culture and Research (ACECR) , Ili ، Pinar Department of Medical Services and Techniques - Denizli Vocational School of Health Services - Pamukkale University , Gul ، Murat Department of Urology - Selcuk University School of Medicine , Ashrafzade ، Amir Mahdi Department of Genetics - Royan Institute for Reproductive Biomedicine, Reproductive Biomedicine Research Center - Academic Center for Education, Culture and Research (ACECR) , Kazem-Allilo ، Asghar Buffalo Breeding and Training Extension Center , Garip ، Mustafa Department of Animal Science - Faculty of Veterinary Medicine - Selcuk University , Shahverdi ، Abdolhossein Department of Embryology - Royan Institute for Reproductive Biomedicine, Reproductive Biomedicine Research Center - Academic Center for Education, Culture and Research (ACECR)
Abstract :
Background: The purpose of this research was to compare the functional parameters of frozenthawed Iranian Azari buffalo spermatozoa with imported semen samples of Italian Mediterranean buffalo (IMB) after the thawing process and 4 hours of incubation. Materials and Methods: : In this experimental study, a total of twenty-four ejaculates from four Iranian Azari buffalo bulls were collected. Semen samples were diluted in AndroMed extender at a concentration of 50×106 spermatozoa/ ml. The diluted samples were filled in 0.5 ml straws and were frozen in a programmable freezer. For imported semen samples, twenty-four samples of four IMB were used, which were diluted in AndroMed extender and frozen by the same procedure. Frozen-thawed sperm motion patterns, mitochondrial activity, membrane integrity, DNA integrity, reactive oxygen species (ROS), and apoptosis status were evaluated immediately after thawing and 4 hours of incubation. Results: Post-thawed sperm motility, progressive motility (PM), mitochondrial activity, membrane integrity were significantly higher in imported semen samples in compare with Iranian Azari buffalo. After 4 hours of incubation, sperm velocity patterns were superior in Iranian Azari semen samples. Moreover, the percentage of sperm cells with un-damaged DNA was higher in Iranian semen samples compared to imported samples at the time 0 of incubation. Following 4 hours of incubation, a significant increase in intracellular ROS level leads to reduced membrane integrity, mitochondrial activity, and DNA integrity in both buffalo breeds. At time 4, Iranian samples showed significantly lower apoptosis and higher dead spermatozoa compared to imported semen samples. Conclusion:Our study showed that the post-thawed quality of Iranian Azari buffalo semen was comparable with imported samples after 4 hours of incubation. Further investigations are recommended to assess the in vitro and in vivo fertility rate of both buffalo breeds.
Keywords :
Buffalo , Flow Cytometric Analysis , Iranian Azari Buffalo Breed , Italian Mediterranean Buffalo Breed , Sperm Cryopreservation
Journal title :
International Journal of Fertility and Sterility
Journal title :
International Journal of Fertility and Sterility
