Author/Authors :
Omosun, YO Bells University of Technology - College of Food Sciences - Department of Biotechnology, Nigeria , Adoro, S University of Pennsylvania - Immunology Graduate Group, USA , Anumudu, CI University of Ibadan - Department of Zoology, Cellular Parasitology Programme, Nigeria , Odaibo, A University of Ibadan - Department of Zoology, Cellular Parasitology Programme, Nigeria , Holder, AA National Institute for Medical Research, UK , Nwagwu, M University of Ibadan - Department of Zoology, Cellular Parasitology Programme, Nigeria , Nwuba, RI University of Ibadan - Department of Zoology, Cellular Parasitology Programme, Nigeria
Abstract :
Background: Some MSP-1 19 specific antibodies that inhibit merozoite invasion also inhibit the secondary processing of MSP-1. However the binding of these inhibitory antibodies can be blocked by another group of antibodies, called blocking antibodies, which recognize adjacent or overlapping epitopes, but themselves have no effect on either MSP-1 processing or merozoite invasion. These antibodies have been reported to be present in individuals living in a malaria endemic area. Methods: Blood samples were obtained from children shown to have processing inhibitory, blocking, and neutral antibodies in a previous study. Enzyme linked immunosorbent assay (ELISA), was used to determine the total IgG, IgM and IgG subtypes. Results: There was a significant difference in anti-MSP-1 19 IgG, while there was no significant difference in the anti-MSP-1 19 IgM. Only anti MSP-1 19 IgG1, amongst the IgG subtypes was significantly different between the groups. Conclusion: This study shows that antibodies against MSP-1 are different not only in specificity and function but also in the amount of total IgG and IgG subtype produced.
Keywords :
IgG subtypes , MSP , 1 , malaria
