Author/Authors :
Olasehinde, GI Covenant University - College of Science and Technology - Department of Biological Sciences, Nigeria , Yah, CS National Institute for Occupational Health (NIOH) - Toxicology and Biochemistry Section, South Africa , Singh, R National Institute of malaria Research - Molecular Biology Laboratories, India , Ojuronbge, O Olusola Ladoke Akintola University of Technology - Department of Medical Microbiology and Parasitology, Nigeria , Ajayi, AA Covenant University - College of Science and Technology - Department of Biological Sciences, Nigeria , Valecha, N National Institute of malaria Research - Molecular Biology Laboratories, India , Abolaji, AO Covenant University - College of Science and Technology - Department of Biological Sciences, Nigeria , Adeyeba, AO Ladoke Akintola University of Technology - Department of Medical Microbiology and Parasitology, Nigeria , olasehinde, g.i. department of biological sciences,college of science and technology,covenant university, Nigeria , yah, c.s. toxicology and biochemistry section,national institute for occupational health,25 hospital road,constitutional hill, South Africa , singh, r. molecular biology laboratories,national institute of malaria research,dwarka, India , olusola, o.o. department of medical microbiology and parasitology,ladoke akintola university of technology, Nigeria , ajayi, a.a. department of biological sciences,college of science and technology,covenant university, Nigeria , valecha, n. molecular biology laboratories,national institute of malaria research,dwarka, India , abolaji, a.o. department of biological sciences,college of science and technology,covenant university, Nigeria , adeyeba, a.o. department of medical microbiology and parasitology,ladoke akintola university of technology, Nigeria
Abstract :
Background: Plasmodium falciparum the main causative agent of malaria is an important public health vector. With the use of PCR, its genetic diversity has been extensively studied with dearth information from Nigeria. Methods: In this study, 100 P. falciparum strains merozoite surface protein 1( msp-1), merozoite surface protein 2 (msp-2) and Glutamate rich protein (Glurp) from Ogun State General Hospitals were characterized. The genetic diversity of P. falciparum isolates was analyzed by restriction fragment length polymorphism following gel electrophoresis of DNA products from nested polymerase chain reactions (PCR) of their respective allelic families KI, MAD 20, RO33 (MSP-1);FC27, 3D7 (MSP-2) and Glutamate rich protein respectively. Results: Majority of the patients showed monoclonal infections while multiplicity of the infection for msp-1 and msp-2 were 1.1 and 1.2 respectively. The estimated number of genotypes was 8 msp-1 (4 KI; 3 MAD; 1 RO33) and 6 msp-2 (3 FC27; 3 3D7). 80% of the isolates coded for Glurp with allelic size ranged between 700 and 900 bp. Conclusion: The allelic distributions however were similar to those previously reported in other endemic malaria countries. Future studies will be designed to include other malaria endemic regions of Nigeria such as the oil exploration regions.
