مرکز منطقه ای اطلاع رساني علوم و فناوري - Comparative anti-inflammatory properties of Capsaicin and ethyl-aAcetate extract of Capsicum frutescens linn [Solanaceae] in rats

Author/Authors :

Jolayemi, AT Australia National University (ANU), Goulburn Base Campus - Goulburn Base Hospital - Department of Anaesthesia and ICU, Australia , Ojewole, JAO University of KwaZulu Natal - Faculty of Health Sciences - Department of Pharmacology, South Africa , jolayemi, a.t. department of anaesthesia and icu,australia national university (anu)/goulburn base hospital,australia national university (goulburn base campus), Australia , ojewole, j.a.o. department of pharmacology,university of kwazulu, South Africa

Abstract :

Background: The analgesic effect of capsaicin (the active ingredient in Capsicum frutescens Linn. [Solanaceae]) had been reported in several studies. Current research is being directed at producing analgesics, anti-inflammatory agents with better side effect profile. Objectives: To investigate if either the ethyl acetate extract of Capsicum frutescens Linn. [Solanaceae] (CFE) or capsaicin (Fluka Biotechnika-CPF) (in addition to the known analgesic properties) has any anti-inflammatory effect comparable to non-steroidal anti-inflammatory analgesics (NSAIDS). Methods: The effects of ethyl acetate extract of Capsicum frutescens Linn. [Solanaceae] (CFE) and capsaicin (Fluka Biotechnika- CPF) was examined on rat hind paw. Inflammation was induced in the rat’s hind paw by subplantar injections of fresh egg albumin (0.5 ml/kg). Diclofenac (100 mg/kg) was used as the reference anti-inflammatory agent for comparison, while distilled water was used as the placebo. The leucocytes count, corticosterone and C - reactive protein (CRP) levels were measured as biomarkers of inflammation. Data obtained were pooled and analysed using repeated ANOVA, in a general linear model with the CPSS software. Results: Sub-plantar injections of fresh egg albumin (0.5 ml/kg) produced profound and time-related oedema in the rat hind paw of the ‘control’ rats. Diclofenac (DIC, 100 mg/kg, i.p.) and reference capsaicin (CPF, 2.5 mg/kg, i.p.) significantly inhibited paw swelling at (p 0.05–0.001) (CI 95%) compared to distilled water-treated ‘controls’. While the corticosterone levels were all very low in 7 rats treated with capsaicin, the leucocytes count was within normal range in 9 rats. However, in 16 specimens randomly assigned for CRP levels, there were very high CRP readings, up to a magnitude of 10 times the normal range. Conclusion: Capsaicin in both forms (CFE and CPF) produced anti-inflammatory effects that were comparable to diclofenac in the experimental rat model at p 0.05. It may be concluded that capsaicin has both analgesic and anti-inflammatory properties.