Author/Authors :
Zhao, Huihui Beijing University of Chinese Medicine, China , Li, Zhigeng China Academy of Chinese Medical Sciences - Institute of Basic Theory, China , Tian, Guihua Beijing University of Chinese Medicine - Dongzhimen Hospital, China , Gao, Kuo Beijing University of Chinese Medicine, China , Zhiyong, Li China Minority Traditional Medical Center, China , Zhao, Baosheng Beijing University of Chinese Medicine, China , Wang, Juan Beijing University of Chinese Medicine, China , Luo, Liangtao Beijing University of Chinese Medicine, China , Pan, Qiu Beijing University of Chinese Medicine, China , Zhang, Wenting Beijing University of Chinese Medicine, China , Wu, Zhiqian Beijing University of Chinese Medicine, China , Chen, Jianxin Beijing University of Chinese Medicine, China , Wang, Wei Beijing University of Chinese Medicine, China
Abstract :
Background: Type II diabetes has become a serious threat to human health in recent years. Among adults above 20 years old in China, the prevalence rate of diabetes is 9.7%. Thus, it is imperative to study the mechanisms underlying type II diabetes to develop effective therapeutic treatments. Objective: To examine metabolic changes in a rat model of type II diabetes and explore mechanisms underlying the beneficial effects of traditional Chinese medicine (TCM) in this model. 120 rats were divided into four groups, including a control group, a high-fat diet group (high-fat diet and streptozotocin injection), a TCM group (high-fat diet, streptozotocin injection, followed by TCM administration), and a rosiglitazone maleate group (high-fat diet, streptozotocin injection, followed by rosiglitazone maleate administration). Metabolites in urine samples from 1-3 weeks (time point 1) and 4-6 weeks (time point 2) of drug administration were compared by gas chromatography-mass spectrometry (GC-MS). Results: Our results showed that in the high-fat diet group, at time point 2, the levels of dihydroxybenzoic acid, L-ascorbic acid, D-gluconic acid, octadecanoic acid, and glutaric acid in urine were significantly higher than at time point 1. In the TCM group, at time point 2, the urine levels of L-ascorbic acid were markedly lower than at time point 1. Conclusion: Our studies demonstrated that examining urine metabolic changes provided important insights into the mechanisms underlying type II diabetes as well as the therapeutic effects of TCM.
Keywords :
Type II diabetes , Urine metabonomics , Traditional Chinese Medicine , Rat Model
