Author/Authors :
Gao, Chengcheng Nanjing Medical University - Huai’an First People’s Hospital - Department of Gastroenterology, China , Ma, Tianheng Nanjing Medical University - Huai’an First People’s Hospital - Department of Gastroenterology, China , Pang, Liqun Nanjing Medical University - Huai’an First People’s Hospital - Department of Gastroenterology, China , Xie, Rui Nanjing Medical University - Huai’an First People’s Hospital - Department of Gastroenterology, China , gao, c. department of gastroenterology,huai an first people s hospital,nanjing medical university,6 beijing road west,huai an, China , ma, t. department of gastroenterology,huai an first people s hospital,nanjing medical university,6 beijing road west,huai an, China , pang, l. department of gastroenterology,huai an first people s hospital,nanjing medical university,6 beijing road west,huai an, China , xie, r. department of gastroenterology,huai an first people s hospital,nanjing medical university,6 beijing road west,huai an, China
Abstract :
Background: Herbs might affect the homeostasis of bile acids through influence of multiple metabolic pathways of bile acids. Objective: To investigate the inhibition of cryptotanshinone towards the glucuronidation of LCA, trying to indicate the possible influence of cryptotanshinone-containing herbs towards the homeostasis of bile acids. Methods: The LCA-3-glucuronidation and LCA-24-glucuronidation reaction was monitored by LC-MS. Results: Initial screening showed that 100 μM of cryptotanshinone inhibited LCA-24-glucuronidation and LCA-3-glucuronidation reaction activity by 82.6% and 79.1%, respectively. This kind of inhibition behaviour exerted cryptotanshinone concentrations-dependent and LCA concentrations-independent inhibition behaviour. Conclusion: All these data indicated the possibility of cryptotanshinone’s influence towards bile acids metabolism and homeostasis of bile acids.
