Author/Authors :
Adegbolagun, Olayemi M University of Ibadan - Faculty of Pharmacy - Department of Pharmaceutical Chemistry, Nigeria , Emikpe, Benjamin O University of Ibadan - Faculty of Veterinary Medicine - Department of Veterinary Pathology, Nigeria , Woranola, Ibukun Oluwa O University of Ibadan - Faculty of Pharmacy - Department of Pharmaceutical Chemistry, Nigeria , Ogunremi, Yetunde University of Ibadan - Faculty of Pharmacy - Department of Clinical Pharmacy and Pharmacy Management, Nigeria , adegbolagun olayemi, m. department of pharmaceutical chemistry,university of ibadan, Nigeria , emikpe benjamin, o. department of veterinary pathology,university of ibadan, Nigeria , woranola ibukun oluwa, o. department of pharmaceutical chemistry,university of ibadan, Nigeria , yetunde, o. department of clinical pharmacy and pharmacy management,university of ibadan, Nigeria
Abstract :
Background: Resistance to most antimalarial drugs has encouraged the use of herbal preparations along with prescribed orthodox drugs. Objective: this study investigated effect of co-administration of aqueous extract of T. occidentalis leaves, commonly used as antimalarial and haematinic agent in Nigeria, and artesunate using P. berghei animal model. Methods: In vivo curative antiplasmodial effect of T. occidentalis (200mg/kg) alone and in combination with artesunate (2mg/kg) were evaluated using albino mice infected with 106 parasitized erythrocytes of P. berghei intraperitoneally. The haematological parameters: haemoglobin level, red blood cells and white blood cells and packed cell volume were monitored using standard methods. Results: Aqueous extract of T. occidentalis, artesunate and the combination gave 72.17±4.07%, 70.43± 4.27% and 85.43± 3.65% reduction in parasitaemia after 48hours respectively. A significant enhancement of the PCV was obtained with the co-administration of artesunate and aqueous extract (p 0.01). Similar trends were also observed with heamatological parameters at 72 hours of administration. Conclusion: This study revealed a synergistic effect of the co-administration on parasite clearance rate of P. berghei infection in mice, with a significant enhancement of haematological parameters within 48 hours of administration. This indicates a rapid rate of recovery from plasmodial infections with the co-administration.
