POLYMORPHISM OF ANGIOTENSIN II TYPE 1RECEPTOR GENE IN ESSENTIAL HYPERTENSION IN IRANIAN POPULATION

Renin angiotensin system (RAS) has an important role in the regulation of hypertension. RAS includesangiotensinogen, Angiotensin Converting Enzyme (ACE), angiotensine II and angiotensin receptors(AGTR). Angiotensin receptors have several types but AT1R is the main subtype. In this study the effectof A1166→C polymorphism of AT1R gene and the role of possible genetic differences in hypertensionwas investigated. DNA of the whole blood leukocytes from hypertensive patients and healthy people ofMashhad population as control, were extracted and then PCR was performed on prepared samplesfollowed by amplification of the target fragments which were then digested with the DdeI restrictionenzyme. Data were classified on the basis of genotypes and gender and then alleles and genotypesfrequencies were analyzed statistically. There were no significant differences in the genotype, and allelefrequencies between hypertensive and normotensive subjects. However, frequency of C allele of AT1Rgene in hypertensive women was significantly higher than normotensive women (P 0.05). These resultssuggest that C allele of AT1R gene may be an important risk factor for essential hypertension in women.