Title of article :
DETECTION OF HUMAN PAPILOMAVIRUS TYPES 16 AND 18 IN PATHOLOGIC SAMPLES FROM PATIENTS WITH CERVICAL CANCER BY PCR AND RFLP METHODS
Author/Authors :
MALEKNEJAD, PARVIZ tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , RAKHSHAN, MOHAMMAD shahid beheshti university of medical sciences - Department of Pathology, تهران, ايران , KAZEMI, BAHRAM shahid beheshti university of medical sciences - School of Medicine - Department of Parasitology, تهران, ايران , FAROKH, FARNAZ tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران , SHAHSAVAN, SHADI tehran university of medical sciences tums - School of Medicine - Department of Microbiology, تهران, ايران
Abstract :
Infection with human papiloma virus (HPV) is the most frequent sexually transmitted disease worldwide.HPV types 16, 18, 31 and 33 are considered as the most important types in the cervical cancer.This studywas undertaken on 64 samples of archival cervical carcinoma pathologic to assess the rate of HPVinfection (HPV16,18) in cervical carcinoma among Iranian patients. HPV DNA was detected bypolymerase chain reaction (PCR) and typing by restriction fragment length polymorphism (RFLP)analysis.The total prevalence of HPV in this study (HPV16,18) for all cases was 59.4% (38/64). HPVtype 16 was the most common one (22/64, 34%) followed by HPV type 18 (16/64, 25%). On the basis of the rate of HPV (16,18) which were detected in squamous cell carcinoma and adenocarcinoma,onlywomen with HPV18 infection showed a statistically significant risk for development of cervical cancer(P=0.019) while P value for HPV16 was 0.47 .
Keywords :
HPV , Cervical cancer , PCR , Iran
Journal title :
Daru Journal of Pharmaceutical Sciences
Journal title :
Daru Journal of Pharmaceutical Sciences
