Author/Authors :
Abbasi, M tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Akbari, M tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Amidi, F tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Ragerdi Kashani, I tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Mahmoudi, R yasuj university of medical sciences, ايران , Sobhani, A tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Takzare, N tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , pasbakhsh, P tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Barbarestani, M tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Abolhassani, F tehran university of medical sciences tums - Department ofAnatomy, تهران, ايران , Sato Tohoko University - Graduate School of Agriculture Science, Japan
Abstract :
Background: Nitric oxide (NO) have a dual action in mouse oocyte meiotic maturation which depends on its concentration, but the mechanisms by which it influences oocyte maturation has not been exactly clarified. In this study different signaling mechanisms which exist for in vitro maturation of meiosis was examined in cumulus cell-enclosed oocytes (CEOs) after injection ofpregnant mare s serum gonadotropin (PMSG) to immature female mice. Methods: The CEOs were cultured in spontaneous maturation and hypoxanthine (HX) arrested model. Results: Sodium nitroprusside (SNP, an NO donor, 10mM) delayed germinal vesicle breakdown (GVBD) significantly during the first 5 hrs of incubation and inhibited the formation of first polar body (PB1) at the end of 24 hrs of incubation. SNP (10-5M) stimulated the meiotic maturation of oocytes significantly by overcoming the inhibition of HX. Sildenafil (a cGMP stimulator, 100 nM), had a significant inhibitory effects on both spontaneous meiotic maturation and HX-arrested meiotic maturation. Forskolin (an adenylate cyclase stimulator, 6JlM) and SNP (10mM) had the same effects on GVBD. Forskolin reversed the SNP (10-5M) stimulated meiotic maturation. Conclusion: These results suggest that differences in pathways are present between SNPinhibited spontaneous meiotic maturation and SNP-stimulated meiotic maturation in mouse oocytes
Keywords :
Nitric oxide , Sildenafil , Oocytes maturation , Signaling pathway
