Author/Authors :
Mohamadipoor, M Iranian Blood Transfusion Organization - Research Center, ايران , Habibi Roudkenar, M Iranian Blood Transfusion Organization - Research Center, ايران , Masroori, N Iranian Blood Transfusion Organization - Research Center, ايران , Mohammadi Roushandeh, A tabriz university of medical sciences - Department ofAnatomy, تبريز, ايران , Saki, S islamic azad university - Department 0 [Biology, ايران
Abstract :
Background and the purpose ~f the study: One of the major concerns about recombinant protein production is its possible toxicity for the organism. Purification of the recombinant protein is another challenge in this respect. Recently In Vitro translation cell free system that provides a coupled transcription-translation reaction for protein synthesis to overcome the above mentioned problems has been emerged. The aim of this study was expression of GFP as a marker for gene expression and protein in In Vitro translation system. Methods: pIVEX2.3-GFP plasmid was cloned to E. coli and the plasmid DNA extracted. In Vitro translation was performed with RTS 100 E. coli Hy kit according to manufacture s instructions. Expression of recombinant fusion protein, His- GFP, was determined by SDS-PAGE, ELISA and western blot analysis. Results: Expected size of recombinant protein was detected in SDS-PAGE and further confirmed by western blot analysis and ELISA. A/ajar conclusion: Results showed that In Vitro translation is suitable for expression of recombinant protein and fusion of the recombinant protein with His-tag facilitates the purification.
