Author/Authors :
Moazeni, Esmaeil tehran university of medical sciences tums - School of Pharmacy - Department of Pharmaceutics, Aerosol Research Laboratory, تهران, ايران , Gilani, Kambiz tehran university of medical sciences tums - School of Pharmacy - Department of Pharmaceutics, Aerosol Research Laboratory, تهران, ايران , Rouholamini Najafabadi, Abdolhossein tehran university of medical sciences tums - School of Pharmacy - Department of Pharmaceutics, Aerosol Research Laboratory, تهران, ايران , Rouini, Mohamad reza tehran university of medical sciences tums - School of Pharmacy - Department of Pharmaceutics, تهران, ايران , Mohajel, Nasir tehran university of medical sciences tums - School of Pharmacy - Department of Pharmaceutics, Aerosol Research Laboratory, تهران, ايران , Amini, Mohsen tehran university of medical sciences tums - School of Pharmacy, Drug Design and Development Research Center - Department of Medicinal Chemistry, تهران, ايران , Barghi, Mohammad Ali university of tehran - School of Sciences - XRD Research Laboratory, تهران, ايران
Abstract :
Background: This study evaluated the potential of chitosan based polymeric micelles as a nanocarrier system for pulmonary delivery of itraconazole (ITRA). Methods: Hydrophobically modified chitosan were synthesized by conjugation of stearic acid to the hydrophilic depolymerized chitosan. FTIR and 1HNMR were used to prove the chemical structure and physical properties of the depolymerized and the stearic acid grafted chitosan. ITRA was entrapped into the micelles and physicochemical properties of the micelles were investigated. Fluorescence spectroscopy, dynamic laser light scattering and transmission electron microscopy were used to characterize the physicochemical properties of the prepared micelles. The in vitro pulmonary profile of polymeric micelles was studied by an air-jet nebulizer connected to a twin stage impinger. Results: The polymeric micelles prepared in this study could entrap up to 43.2±2.27 μg of ITRA per milliliter. All micelles showed mean diameter between 120–200 nm. The critical micelle concentration of the stearic acid grafted chitosan was found to be 1.58×10-2 mg/ml. The nebulization efficiency was up to 89% and the fine particle fraction (FPF) varied from 38% to 47%. The micelles had enough stability to remain encapsulation of the drug during nebulization process. Conclusions: In vitro data showed that stearic acid grafted chitosan based polymeric micelles has a potential to be used as nanocarriers for delivery of itraconazole through inhalation.
Keywords :
Polymeric micelles , Itraconazole , Chitosan , Nebulization , Pulmonary drug delivery
