Author/Authors :
Barazandeh Tehrani, Maliheh tehran university of medical sciences tums - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Department of Medicinal Chemistry, تهران, ايران , Namadchian, Melika tehran university of medical sciences tums - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Department of Medicinal Chemistry, تهران, ايران , Fadaye Vatan, Sedigheh Kish Medipharm Company - R D Department, ايران , Souri, Effat tehran university of medical sciences tums - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Department of Medicinal Chemistry, تهران, ايران
Abstract :
A derivative spectrophotometric method was proposed for the simultaneous determination of clindamycin and tretinoin in pharmaceutical dosage forms. The measurement was achieved using the first and second derivative signals of clindamycin at (1D) 251 nm and (2D) 239 nm and tretinoin at (1D) 364 nm and (2D) 387 nm. The proposed method showed excellent linearity at both first and second derivative order in the range of 60–1200 and 1.25–25 μg/ml for clindamycin phosphate and tretinoin respectively. The within-day and between-day precision and accuracy was in acceptable range (CV 3.81%, error 3.20%). Good agreement between the found and added concentrations indicates successful application of the proposed method for simultaneous determination of clindamycin and tretinoin in synthetic mixtures and pharmaceutical dosage form.
Keywords :
Clindamycin , Tretinoin , Derivative spectrophotometry , Pharmaceutical dosage form
