Title of article :
Quantification of verbascoside in medicinal species of Phlomis and their genetic relationships
Author/Authors :
Sarkhail, Parisa tehran university of medical sciences tums - Pharmaceutical Sciences Research Center, تهران, ايران , Nikan, Marjan tehran university of medical sciences tums - Faculty of Pharmacy, Medicinal Plants Research Center, تهران, ايران , Sarkheil, Pantea tehran university of medical sciences tums - Pharmaceutical Sciences Research Center, تهران, ايران , Gohari, Ahmad R tehran university of medical sciences tums - Faculty of Pharmacy, Medicinal Plants Research Center, تهران, ايران , Gohari, Ahmad R University of Saskatchewan - College of Pharmacy and Nutrition - Division of Pharmacy, Canada , Ajani, Yousef Culture and Research (ACECR) - Institute of Medicinal Plants (IMP), Iranian Academic Centre for Education, ايران , Hosseini, Rohollah tehran university of medical sciences tums - Faculty of Pharmacy - Department of Toxicology and Pharmacology, تهران, ايران , Hadjiakhoondi, Abbass tehran university of medical sciences tums - Faculty of Pharmacy, Medicinal Plants Research Center - Department of Pharmacognosy, تهران, ايران , Saeidnia, Soodabeh tehran university of medical sciences tums - Faculty of Pharmacy, Medicinal Plants Research Center, تهران, ايران , Saeidnia, Soodabeh University of Saskatchewan - College of Pharmacy and Nutrition - Division of Pharmacy, Iran.
From page :
1
To page :
9
Abstract :
Background: The genus Phlomis (Lamiaceae) is introduced by its valuable medicinal species, of which 17 species are growing wildly and ten of them are exclusively endemic of Iran. The main phytochemical characteristic of this genus is presence of iridoid glycosides including ipolamide, auroside, lamiide and also phenylethanoids such as verbascoside (acetoside) found in Lamiales order. Due to the broad range of biological and pharmacological activities of verbascoside and lack of any report on quantification of this compound within Iranian species of Phlomis, we conducted a research to achieve two main goals, finding a genetic biodiversity by RAPD (Randomly Amplified Polymorphic DNA), as well as detecting and quantifying verbascoside in nine species of Phlomis growing wildly in Iran. Results: The results showed that various samples of P.olivieri possess different genetic distances from each other. Also, various species of P.olivieri display close relationships to P.anisodonta and P. persica. Phytoanalysis of Phlomis species by means of TLC scanner using verbascoside as a phytochemical marker showed that the highest concentration of verbascoside was found in P. anisodonta, however, P. bruguieri and P. olivieri (from Mazandaran) were in the second and third places. Interestingly, the lowest concentration of verbascoside was detected in P. olivieri (from Azerbayjan), exhibiting the effect of various growing areas and conditions on the measured levels of this compound. Conclusions: verbascoside can be found in various species of Iranian Phlomis, of which P. anisodonta, P. bruguieri and P. olivieri might be the best choices. In addition, although the concentration of verbascoside in these plants may be affected by the growing areas and conditions, there are a good agreement between genetic relations and verbascoside levels.
Keywords :
Phlomis , RAPD , Verbascoside , Quantification , TLC scanner
Journal title :
Daru Journal of Pharmaceutical Sciences
Journal title :
Daru Journal of Pharmaceutical Sciences
Record number :
2634886
Link To Document :
بازگشت