Author/Authors :
Esfandyari-Manesh, Mehdi tehran university of medical sciences tums - Nanotechnology Research Centre, Faculty of Pharmacy - Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران , Mostafavi, Hossein tehran university of medical sciences tums - Faculty of Pharmacy , School of Advanced Technologies in Medicine - Medical Nanotechnology Department, Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران , Mostafavi, Hossein University of California - Department of Bioengineering, USA , Faridi Majidi, Reza tehran university of medical sciences tums - School of Advanced Technologies in Medicine - Medical Nanotechnology Department, تهران, ايران , Noori Koopaei, Mona tehran university of medical sciences tums - Faculty of Pharmacy - Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران , Shabani Ravari, Nazanin tehran university of medical sciences tums - Nanotechnology Research Centre, Faculty of Pharmacy - Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران , Amini, Mohsen tehran university of medical sciences tums - Faculty of Pharmacy - Department of Medicinal Chemistry, تهران, ايران , Darvishi, Behrad tehran university of medical sciences tums - Nanotechnology Research Centre, Faculty of Pharmacy, School of Advanced Technologies in Medicine - Medical Nanotechnology Department, تهران, ايران , Ostad, Nasser tehran university of medical sciences tums - Faculty of Pharmacy and Pharmaceutical Sciences Research Center - Department of Toxicology and Pharmacology, تهران, ايران , Atyabi, Fatemeh tehran university of medical sciences tums - Nanotechnology Research Centre, Faculty of Pharmacy - Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران , Dinarvand, Rassoul tehran university of medical sciences tums - Faculty of Pharmacy, Nanotechnology Research Centre - Department of Pharmaceutics, Novel Drug Delivery Lab, تهران, ايران
Abstract :
Background: Nanoparticles (NPs) play an important role in anticancer delivery systems. Surface modified NPs with hydrophilic polymers such as human serum albumin (HSA) have long half-life in the blood circulation system. Methods: The method of modified nanoprecipitation was utilized for encapsulation of paclitaxel (PTX) in poly (lactic-co-glycolic acid) (PLGA). Para-maleimide benzoic hydrazide was conjugated to PLGA for the surface modifications of PLGA NPs, and then HSA was attached on the surface of prepared NPs by maleimide attachment to thiol groups (cysteines) of albumin. The application of HSA provides for the longer blood circulation of stealth NPs due to their escape from reticuloendothelial system (RES). Then the physicochemical properties of NPs like surface morphology, size, zeta potential, and in-vitro drug release were analyzed. Results: The particle size of NPs ranged from 170 to 190 nm and increased about 20–30 nm after HSA conjugation. The zeta potential was about -6 mV and it decreased further after HSA conjugation. The HSA conjugation in prepared NPs was proved by Fourier transform infrared (FT-IR) spectroscopy, faster degradation of HSA in Differential scanning calorimetry (DSC) characterization, and other evidences such as the increasing in size and the decreasing in zeta potential. The PTX released in a biphasic mode for all colloidal suspensions. A sustained release profile for approximately 33 days was detected after a burst effect of the loaded drug. The in vitro cytotoxicity evaluation also indicated that the HSA NPs are more cytotoxic than plain NPs. Conclusions: HSA decoration of PLGA NPs may be a suitable method for longer blood circulation of NPs.
