EXTRACTION AND PURIFICATION OF RESVERATROL FROM GRAPE SKIN FRUIT VITIS VINIFERA

This study was conducted with the aim to extract and purify a polyphenolic compound “Resveratrol” from the skin of black grapes Vitis vinifera cultivated in Iraq. The partial purified resveratrol is obtained after chromatography on silica gel G60 column. The preperative thin layer chromatography elution yields pure crystals identified as resveratrol (mixture of the two isomers cis and trans ) in relation to resveratrol standard trans- resveratrol (35 mg resveratrol crystals/0.5kg fresh grape skin obtained as a result of these processes). Chemical investigations and tests for identification and qualification of the extracted partial and purified crystals involved: general tests for polyphenoles, aromatic unsaturated compounds, spectrophotometric scanning for λmax screening, High Performance Liquid Chromatography (HPLC) analysis, Fourier Transform Infra-Red (FTIR) assay and melting point estimation in relation to resveratrol standard for the all applied tests.