Title of article :
LIPOFECATION OF THE HUMAN LARYNX CARCINOMA CELL LINE (HEP-2) WITH PUC18 PLASMID USING TRANSFAST REAGENT AS A LIPOSOME
Author/Authors :
Salo, Wisam H. Baghdad University - Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, Iraq , Oleiwi, Abdul- Rahman A. Baghdad University - Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, Iraq , Moneer, Ali I. Baghdad University - Institute of Genetic Engineering and Biotechnology for Postgraduate Studies, Iraq
Abstract :
Here we describe the lipofecation of the human larynx carcinoma cell line (Hep-2) using TransFastTm reagent as a liposome after its mixing with pUC18 plasmid. Optimization of the charge ratio for liposome/DNA was carried out. The DNA was extracted and PCR analysis for the presence of the plasmid is carried out using two specific primers for pUC18. Result indicates that lipofecation with TransFastTm reagent is successful for Hep-2, the optimum charge ratio of transfection reagent to DNA was 1:1, and the optimum DNA concentration was 0.75-1ng.
Keywords :
Lipofecation , Hep , 2 , Transfast , Plasmid.
Journal title :
iraqi journal of biotechnology
Journal title :
iraqi journal of biotechnology
