LACTOBACILLUS PLANTARIUM GE6 REDUCESAFLATOXIN B1 TOXICITY IN RATS AND CACO-2CELLS

Aflatoxin B1 is potent mycotoxin produced by some Aspergillus species, is a hepatocarcinogen and compromise immunity and interferes with protein synthesis and metabolism in human and animals. In this study we investigated the potential of L.plantarium GE 6 to reduce aflatoxin B1 toxicity in rats and DNA fragmentation in Caco-2cells line. This work was conducted on 36 male Han-Wister rats which were divided into 3equal groups each one contain twelve rat,first group received phosphate saline alone and the second group received L.plantarium GE6(3×107CFU/0.5ml phosphate saline) at the fourth day of experiment both groups received a single oral dose of aflatoxin B1 2ppm/kg in 0.5ml DMSO, third group served as untreated control. There was no significant reduction in body weight gain in rat dosed 2ppm/kg of aflatoxin B1 alone in comparing with the second group received aflatoxin B1 and L.plantarium together. Fecal excretion of aflatoxin B1 increased to 16.2% ±(3.1) within first day after aflatoxin B1 dose in group receiving aflatoxin B1 and L.plantarium GE6 compared to the group received aflatoxin B1alone 7.4%±(2.3).Liver enzymes were measured in plasma on the sixth day of the experiment , alanine transaminase (ALT) , Glutamate oxalic transferase (GPT) and Glutamate pyruvate transferase (GOT) increased significantly (p 0.05) to 124.5,184.7 and 101 (IU/L) in group exposure to aflatoxin B1 alone, liver enzymes in group received L.plantarium in addition to aflatoxin B1 was not differ significantly from control group and it was 70.2,92.4 and 49.2(IU/L) for ALT,GPT and GOT respectively.DNA fragmentation was assessed in Caco-2 cells exposed to aflatoxin B1 followed induction of Cytochrome P (CYP3A4),DNA damage occur 28.1%and 52.4% after 24 and 72 hour respectively in cells treated with aflatoxin B1 only ,a significant reduction (p 0.05)in DNA damage observed after 72 hour only at cells coincubated with 3×1010 cell/ml of non viable L.plantarium GE6(36.7%of DNA).