COMPARATIVE STUDY BETWEEN VIBRIO CHOLERAISOLATED FROM PATIENTS AND FROM SURFACEWATER IN IRAQ USING PULS FIELDGEL ELECTROPHORESIS

The current work include the molecular epidemiological study of two types of V. cholera, the clinical isolates (9 isolates) which have been taken from laboratory health center–Baghdad, during 2004-2006 and the environmental isolates(5 isolates), recovered from surface water in Baghdad and some governorates by using two methods, the first one the isolation and electrophoresis of plasmid DNA through Gel electrophoresis, the second, the isolation and electrophoresis of chromosomal DNA using PFGE for the detection source of Cholera infection. Results revealed eight clinical isolates were V. Cholera type O1 and subtype Ogawa and Inaba, only one of them was type non O1, while all the environmental isolates were non OI type, beside, all isolates from O1 belonged to El-Tor type. The results of DNA investigation revealed the absence of DNA plasmid in clinical and environmental isolates.While the chromosomal analysis using PFGE showed normal distribution of cutting chromosomes digested by restriction enzyme not I revealing nine bands of DNA of Cholera subtype Inaba, O1 and Altor type with molecular weight ranging 50-425 kb base. No investigation done for Cholera subtype Ogawa which had been isolated from patient, because it appears like a smear on the gel used.