EXPRESSION OF BAX mRNA IN PATIENTS WITH COLORECTAL CARCINOMAS USING INSITU HYBRIDIZATION

The study investigated the expression of Bax mRNA using in situ hybridization technique in the paraffin sections from 43 biopsies of patients with cancers of colorectal (CRC), 26biopsies from patients with hyperplastic polyps and adenomas(CRHPA) and 35-colon normal tissue (CRN). This investigation correlated the expression patterns with tumor histopathological type, site of the tumor in colorectal cancer patients. Results indicated that in colorectal cancer (CRC) patients’ Bax mRNA expression was detected in 28 of 43. Patients with colorectal cancer were grouped according to the site of the tumor into proximal, distal and rectal site. There was no association between site of tumor and marker detection and no association with the histopathological type. In colorectal hyperplastic polyp and adenoma specimens, Bax mRNA expression was detected in 19 of 26. For the determination of the differences in marker expressions in relation to the histopatholgical type, results showed highly significant in the tubular and villous adenoma expression with P value 0.01. The expression of this marker in relation to the site of the tumor had been studied and showed no significant difference in the expression. In colorectal normal specimens Bax mRNA expression showed significant high expression. While in the (CRN) results were below the used cutoff value for each one of them and regarded as negative. In conclusions, Bax, mRNA expression have high level in CRHPA than in CRN tissue with P value 0.001. Also, its expression in CRC is more than in CRN tissue with a P value 0.001.