HPLC TECHNIQUE FOR ESTIMATION OF CATECHOLAMINE USING AS BIOCHEMICAL BRAIN TUMOR MARKER

High performance liquid chromatography ◌ٌRP-HPLC technique was used to determine the concentration levels of catecholamine (Noradrenaline (NA), Adrenaline (AD) and Dopamine (DA)) in plasma samples of 25 normal volunteer s and 20 brain tumor patients . A chromatographic reversed-phased method based upon ion-pairing has been developed to measure NA, AD and DA on (ODS-DB) column using 50 mM phosphate buffer mixed with 0.5 mM of 1-octane sulphonic acid : Acetonitrile ( 95 / 5)(V/V) pH = 4.5 and monitored on UV-Visible at 280 nm. A different concentrations of standard catecholamine were prepared to get the chromatographic linearity that appeared to be linear in the range 50--10000 ngml. The results show the concentration levels of NA, AD and DA in normal volunteer’s were(256(33)),(269(41)) and (561(61)) ng/ml respectively whereas (274(31)), (256(38)) and (885(73)) ng/ml respectively in brain tumor patients which reflected the presence of a significant increase dopamine compared with normal volunteers which is use as biochemical brain tumor marker.