PHOTODYNAMIC THERAPY OF SUBCUTANEOUS MURINE MAMMARY ADENOCARCINOMA

Photodynamic therapy (PDT) of cancer is a treatment based on the accumulation of a porphyrin-related photosensitizer in tumor cells, and their subsequent destruction based on exposure to light source of specific wave length. Hematoporphyrin derivative has been shown a selective localization in malignant tissues and causing their destruction by generated singlet oxygen when it s activated by appropriate wavelength (λ) of irradiation. Singlet oxygen species are produced and then caused membranes and organelles damage leading to cell death and tumor ablation. In this study, Female mice transplanted with AM3 (mouse mammary adenocarcinoma transplantable tumor line) randomly divided into four groups of 10 mice each, the mice of first group were intratumorally injected with 30 mg HPD/kg of body weight and exposed to 10 minutes of irradiation from 20mW He-Ne laser (λ= 632.8 nm). The mice of second group were intratumorally injected with 30 mg HPD/kg of body weight without irradiation. Third group mice were received 10 minutes exposure time of He-Ne laser irradiation without HPD injection, while the fourth group was leaved as a control. Photodynamic therapy, which includes HPD and irradiation, has had the most powerful effect on the tumor growth, while the other groups have not showed any significant response. With more investigations PDT can be promising anti-breast cancer arsenal.