Detection of Exo A and OPR I Genes in Pseudomonas aeruginosa Using Polymerase Chain Reaction

In this study, two polymerase chain reaction (PCR) assays were performed individually for the molecular detection of Pseudomonas aeruginosa obtained from wound and burn samples. Two gene were targeted as specific markers for P. aeruginosa namely the exotoxin A (exoA) gene and the outer membrane lipoprotein (oprI) gene. Seven isolates for each of P. aeruginosa and other Pseudomonas species were employed in this study and boiled bacterial suspension was used as a DNA template. Results showed that all P. aeruginosa isolates gave positive PCR product for oprI gene with amplified size (196 bp) while only 4isolates were harboring exo A gene with amplified size (396 bp). Results for PCR product for Pseudomonas sp. Revealed that none of the seven isolates were positive for exo A gene and only 4 were positive for opr I gene. This research may indicates that molecular detection of P. aeruginosa from wound and burn samples employing exo A and opr I genes by using PCR technique is a rapid method.