Detection of Enterotoxin (hblA,hblC and hblD ) Genesof Bacillus cereus Isolates from Different FoodSamples Using Traditional and Molecular Methods

A total of 245 samples of foods (pasteurized milk, cheese, yoghurt, meat, and coffee) collected from different local markets at Baghdad city. The samples were cultured on selective media , polymyxin B pyruvate egg yolk mannitol bromothymol blue agar (PEMBA) to isolate B.cereus. Results revealed that the isolation of fifty five B. cereus isolates distributed as 26.2 % in pasteurized milk , 22.8% in cheese, 30.2% in yoghurt, 15.6% in meat and 8% in coffee. To confirm the identification, all the fifty five B.cereus isolates were undergone to biochemical test , and grown on blood agar . PCR amplification resulted in the occurrence of each of the genes hblA, hblC and hblD in the percentages of 38.2%, 60% and 60% respectively out of the total number of isolates.