Title of article :
Production of Antibody (IgG) Against Aflatoxin B1
Author/Authors :
hassan, fadia f. university of baghdad - college of science - department of biology, Iraq , al- jibouri, muna h. university of baghdad - college of science - department of biology, Iraq , mustafa, khatab a. al-sulaimania university - college of science - department of biology, Iraq , hashim, abdul kareem. j. university of baghdad - college of science - departments of biotechnology, Iraq
Abstract :
Aflatoxin B1 (AFB1) is a mycotoxin produced mainly by fungus Aspergillus flavus in food and feed . It is considered as a carcinogenic toxin for human and animals. The current study was designed for produce antibody (IgG) against aflatoxin B1.It was achieved by immunization of experimental animals (New Zealand White rabbits) with prepared antigen consist of aflatoxin B1-BSA Conjugate (100 and 200 μg ) concentrations, and detection of produced antibody using Ouchterlony double immunodiffusion and ELISA techniques,. Ochterlony and ELISA techniques revealed that, high titer of IgG antibody was obtained by rabbit’s immunize, and the titer of antibody was increased steadily during the immunization schedule. The highest titer of antibody reached up to 12800.
Keywords :
Aflatoxin B1 , Antibody , IgG , Ouchterlony double immunodiffusion , ELISA
Journal title :
Iraqi Journal Of Science
Journal title :
Iraqi Journal Of Science
