Histomorphometric Evaluation of Mice Testicular Tissue Fixed by Two Types of Fixatives

In histopathological evaluation, tissue samples are fixed, processed, embedded, and sectioned before being stained. Fixation is the foundation step behind the study of histology and pathology. Routine testicular tissue fixation in 10% formalin solution leads to sever shrinkage which obscures most of the morphological details. The aim of this study was to evaluate the morphological and structural alteration as well as the quantitative variations in size of the seminiferous tubule of mice testes that occur after fixation in either 10% formalin solution or bouin’s solution. The quantitative variations in size of the seminiferous tubule of the testes were performed using Motic image plus version 2.0 ml image analysis system. The quantitative parameters included both area and diameter of equivalent circle (D-Circle). Data showed that the type of the fixative solution affected only on the morphological and structural details of the testicular tissue but there were no significant differences in the quantitative parameters. This study concluded that the testicular tissue fixation with bouin’s solution can be used for better morphological and structural details and the data can be combined with data collected from samples fixed with 10% formaline solution for the histomorphological measurements.