Molecular Detection of AmpC Gene Encoding Antibiotic Resistance among Klebsiella spp. Isolated from Different Infections

One hundred and fifty five of Gram-negative-lactose fermented bacteria grown on MacConkey agar were collected from two main hospitals in Najaf province. The isolates were identified according to cultural characteristics and biochemical activities. The ability of Klebsiellae isolates to AmpC production were tested; the results have revealed that 9 (30%) isolates produce AmpC β-lactamase. PCR amplification results have shown that six Klebsiellae isolates possess AmpC β-lactamase gene.