Title of article :
EXTRACTION OF ATTACHMENT CEMENT CONE PROTEIN FROM LARVAL TICK HAEMAPHYSALIS FLAVA, AS STAGE REACTIVE IMMUNOGEN IN DOMESTIC SHEEP BREED OVIS ARIES BALUCHII FROM BALOCHISTAN, PAKISTAN
Author/Authors :
Iqbal, Asim University of Balochistan - Department of Zoology, Pakistan , Nawaz, Muhammad University of Balochistan - Department of Zoology, Pakistan , Shahabuddin, Mujeebur-Rehman Pakistan Council of Scientific and Industrial Research, Pakistan , Kamran, Kashif University of Balochistan - Department of Zoology, Pakistan , Muhammad, Wali University of Balochistan - Department of Zoology, Pakistan , dastagir, Ghulam University of Balochistan - Department of Zoology, Pakistan
Abstract :
Putative tick cement protein (Haemaphysalin, 30 KDa) was extracted through SDS-PAGE and immunoblotting, from larval attachment cement cone of tick Haemaphysalis flava which elicited a strong stage reactive immune response during larval and post larval stages of tick feeding when parasitized on sheep. During feeding of larvae of ticks the first (30 KDa) protein in the cement cone appeared after SDS-PAGE when probed with anti- Haemaphysalis flava sheep sera collected from immunized hosts. Electroblotted cement proteins of Haemaphysalis flava larvae, nymphs and adults revealed common stage reactive bands of 30 KDa, which proved to be a stage reactive immunogen of importance while immunizing host.
Keywords :
ticks , sheep , SDS , PAGE , immunoblotting, anti , tick vaccine, acarology, tick control
Journal title :
Pakistan Entomologist
Journal title :
Pakistan Entomologist
