Title of article :
Molecular Differentiation of Five Trichogramma Species Occurring in Portugal
Author/Authors :
Silva، Isabel M. M. S. نويسنده , , Honda، Jeffrey نويسنده , , Kan، Frenk van نويسنده , , Hu، Jianguo نويسنده , , Neto.، Luis نويسنده , , Pintureau، Bernard نويسنده , , Stouthamer، Richard نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Abstract :
Two molecular methods were developed to distinguish between five Trichogramma (Hymenoptera, Trichogrammatidae) species which occur naturally in tomato fields of Portugal: Trichogramma cordubensis, T. evanescent T. turkestanica, T. pintoi, and T. bourarachae. The methods used were: (1) electrophoresis of PCR-aniplified ribosomal DNA internal transcribed spacer 2 (ITS-2) followed by restriction endonuclease digestions and (2) esterase electrophoresis. Dichotomous keys based on each of the methods are provided foreasy and quick species differentiation.
Keywords :
T. turkestanica , T. bourarachae , Identification , rDNA internal transcribed spacer , T.pintoi , Trichogramma cordubensis , polymerase chain reaction , esterase electrophoresis , restriction , analysis , Mull , Portugal , T. evanescens
Journal title :
Biological Control
Journal title :
Biological Control
