Molecular identification of methicillin-resistance Staphylococcus aureus isolated from milk of breast infection woman in Diwanyia province.

Methicillin-resistance Staphylococcus aureus (MRSA) is the greatest important source of community-acquired infection in humans. The present study was carried out to use (PCR) assay as more specific molecular methods for recovering of methicillin resistant S.aureus (MRSA) isolates that isolated from milk of breast infection woman which collected from Diwanyia hospital . PCR assay was based on used specific primers that amplified of mecA gene in S.aureus isolates. The primers were designed in this study by using NCBI-Genbank data base (KM505042.1) and primer 3 plus primers design. The results of primary identification bacterial isolation were show 16 positive S.aureus isolates out of 50 milk samples. From those 5(31.25)were identification methicillin-antibiotic-resistant S. aureus (MRSA) using PCR assay The conclusion was to establish a fast and specific PCR tool for the detection of methicillin resistant S. aureus (MRSA)in human milk that may be major risk factor human health.