Evaluation of Actinomycetes Isolates as A Biocontrol Agent Against Spodoptera littoralis (Boisd.) and Fusarium oxysporum

According to soil isolation techniques, 23 isolates of actinomycetes were obtained from different soil samples in KSA and Egypt. All isolates were examined for their biological activity against both, the pest Spodopteralittoralis (Boisd.), also the plant pathogen Fusariumoxysporum. Results illustrated that, three isolates, named DI,DV and FII exhibited high efficacy against the 4th instar of S. littoralis, with mortality percentage 70, 80and 60% respectively. Also, other stages development of S. littoraliswere affected. Meanwhile, metabolites of 20 isolates showed antagonistic activity against F.oxysporum, including the 3 isolates which showed efficacy against the insect pest, the % of inhibition ranged from 67 – 49 %.These 3 isolates were belong to the species Stryptomyceslavendulae and Str. clavuligerus.