Title of article
Diagnosis of human brucellosis in Egypt by polymerase chain reaction
Author/Authors
El Kholy, A.A. University of Cairo - Faculty of Medicine - Department of Clinical and Chemical Pathology, Egypt , Gomaa, H.E. National Research Centre - Department of Clinical and Chemical Pathology, Egypt , El Anany, M.G. University of Cairo - Faculty of Medicine - Department of Clinical and Chemical Pathology, Egypt , Abd El Rasheed, E. National Research Centre - Department of Clinical and Chemical Pathology, Egypt
From page
1068
To page
1074
Abstract
This study aimed to establish an accurate and sensitive polymerase chain reaction (PCR) technique for the diagnosis of active human brucellosis in Egypt. We failed to extract Brucella DNA with a commercial kit, but an extraction kit designed in-house using 2 sets of primers [B4/B5 (223 bp) and JPF/JPR (193 bp)] was successful and more economical. The technique showed high sensitivity, specificity and accuracy. The PCR positivity increased significantly with increasing seropositivity titres by the standard tube agglutination test and showed 100% positivity in patients with positive blood cultures. We recommend using PCR as an alternative to culture for diagnosis of brucellosis.
Journal title
Eastern Mediterranean Health Journal
Journal title
Eastern Mediterranean Health Journal
Record number
2641119
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